Glowing Mushroom biome

A Mushroom biome is a biome consisting of Mushroom Grass on Mud blocks, where Glowing Mushrooms grow, and dangerous enemies spawn. Giant glowing mushrooms can grow to significant heights if room is allowed for them to grow, and will regenerate if chopped down. Most elements of a Mushroom biome emit a faint glow, including its Mushrooms, Grass, and Giant Mushrooms, along with some of its enemies. The Truffle NPC requires a house in a Mushroom biome.

100 tiles of Mushroom Grass are required for a permanent Mushroom Biome.

Surface Mushroom biomes do not generate naturally. They must be created by the player. The sun is removed from Mushroom biomes, making the light level as low as it is at night, even during daytime hours. Their background depicts towering mushrooms. Surface Mushroom biomes can be created by planting Mushroom Grass Seeds in Mud. The Dryad sells Mushroom Grass Seeds if her house resides in a Mushroom biome, or they can drop randomly from harvesting Glowing Mushrooms. In Hardmode, the Clentaminator can also create a Mushroom biome via Dark Blue Solution.

Underground Mushroom biomes are found naturally, randomly generating on world creation anywhere below 0 depth. They can also be created by players. They generally consist of a large cavity with Mud platforms. The default cavern enemies will spawn here, and in Hardmode, more difficult unique enemies spawn. Its background shows numerous small mushrooms. Giant mushrooms will also grow here if there is enough vertical space, though unlike giant mushrooms on the surface, they typically appear without branches.

Their character's high health and damage are still a bit hard to kill in Hardmode.