Guide:Queen Bee strategies

Potions
Get at least 5 Heal Potions.

Armor
Lead armor or higher is enough to do it, although I prefer gold armor.

Accessories
Cloud/Blizzard/Sandstorm in a bottle and a Grappling Hook are very helpful, although not required.

Weapons
Get the best bow you can build and make as many arrows (Fire Arrows) as possible. It is easiest if you have a Bee Gun, although you must have defeated the Queen before to obtain it.

Terrain Preparation
Build a small source of honey to stay in, as it will greatly increase your life regeneration speed. Build dirt over liquids so you move more easily during the battle.

Summon Queen Bee
You can either destroy the Crystallized Larva or craft an Abeemination to summon her.

The Fight
She has 2 attacking moves. She can charge through you and shoot bees at you. Dodge her charge and wait for her to stand still. At this moment you can just spam attacks with your bow or another weapon. If you are low on health, go into the source of honey and use the pickaxe to defend the bees moving onto you (only do this when she stands still.)