Guide:Queen Bee strategies

Potions
Bring at least 5 Healing Potions. Ironskin Potions will also reduce damage, making the fight much easier.

Armor
Crimson armor/Shadow armor or better. If you can't access this armor, try to avoid making contact with, or letting any projectiles touch the larvae until you can acquire better.

Accessories
Cloud/Blizzard/Sandstorm in a Bottle and a Grappling Hook are very helpful, although not required.

Weapons
Get the best bow you can and craft as many arrows (Fire Arrows or Jester Arrows) as possible. Also consider bringing a sword weapon like the blood butcherer, Muramasa or any other large sized swords. It will help when attacking the minion bees. It is easier if you have a Bee Gun, although you must have defeated the Queen before to obtain it. Collecting fallen stars, and using a star cannon, would kill her in about 70 shots. This is fairly effective for she is not immune to knockback.

Terrain Preparation
Build a small source of honey to stay in, as it will greatly increase your life regeneration speed. Build dirt over liquids so you move more easily during the battle. Wood platforms should be placed just above your max jump height (6 blocks) so bees can't hit you.

Summon Queen Bee
You can either destroy the Crystallized Larva (found within hives in the Underground Jungle) or craft an Abeemination to summon her (only in jungle/underground jungle(test greatly please)).

The Fight
She has 3 attacking moves. She can charge through you and shoot bees at you, as well as shoot stingers while flying around. Dodge her charge and wait for her to stand still. At this moment you can just spam attacks with your bow or another weapon. If you are low on health, go into the source of honey and use the pickaxe to defend the bees moving onto you (only do this when she stands still.)