A Glowing Mushroom biome, also known as Glowing Mushroom Fields, is a biome characterized by Mushroom grass, Glowing Mushrooms, and Giant Glowing Mushrooms growing on Mud Blocks. The grasses and plants in a Glowing Mushroom biome emit a vibrant blue glow, along with many of its unique enemies. The Truffle NPC requires a house in a Surface (above-ground) Glowing Mushroom biome and sells mushroom-themed blocks, pets, and weapons.
Underground Glowing Mushroom biomes are Glowing Mushroom biomes below 0 depth. They can be found naturally, randomly generating on world creation. The background of an Underground Glowing Mushroom biome depicts numerous small mushrooms. Several mushroom-themed versions of normal Cavern enemies spawn here in pre-Hardmode, and some completely unique enemies will spawn in Hardmode. Giant Glowing Mushrooms will grow if there is enough vertical space, though unlike Giant Glowing Mushrooms on the surface, they have smaller caps and lack branches. On the Desktop version and Mobile version, Underground Glowing Mushroom biomes are large, layered caverns which feature mushroom "vines" hanging from the ceiling and surface-style Giant Glowing Mushrooms. They generate with set features, including a span of flat ground (for Giant Glowing Mushrooms to grow on) and a wide lake for fishing purposes. These long spans of flat ground are conducive to the spawning of Gold Chests, statues, and other objects which generate underground but rely on having a certain amount of level terrain, making these biomes attractive when hunting for such things.
Surface Glowing Mushroom biomes are Glowing Mushroom biomes above 0 depth. They do not generate naturally and must instead be created by the player. The sky is darkened in Surface Glowing Mushroom biomes, making it appear to be nighttime even during daytime hours. The background depicts towering mushrooms. There are several exclusive enemies, some of which can also be encountered in Underground Glowing Mushroom biomes.
A Glowing Mushroom biome can be created manually by planting Mushroom Grass Seeds on Mud Blocks, or spraying the Jungle with the Clentaminator using Dark Blue Solution. An area requires 101 tiles that are mushroom-related (Mushroom grass, naturally grown Glowing Mushrooms, Giant Glowing Mushrooms) to be considered a Glowing Mushroom biome.
NPC happiness 
|Glowing Mushroom biome|
For more information, see NPC happiness.
- Mushroom grass is immune to being converted by an evil biome, while the interior Mud Blocks are not.
- Use of the Clentaminator with Green Solution on a Glowing Mushroom biome converts it to a Jungle biome. However, attempting to use Purification Powder to convert a Glowing Mushroom biome to a Jungle biome will fail.
- Underground Glowing Mushroom biomes do not generate within the Ice biome and the Underground Desert.
- While the area functionally changes to a Glowing Mushroom biome at 101 related blocks, the background of a Glowing Mushroom biome will not appear until 201 Glowing Mushroom biome blocks are present.
- The Funkytown achievement requires 200 Glowing Mushroom biome blocks nearby the player.
- The Funkytown achievement can sometimes be obtained by using a Magic Mirror, Ice Mirror, or Cell Phone while in an Underground Glowing Mushroom biome. This is likely a bug caused by lag.
- An efficient way to create an artificial Glowing Mushroom biome is to put Mud Blocks in such a fashion that they form a grid of 1×1 empty cells. This way, the Mushroom grass has more blocks it can spread to, greatly accelerating the process of creating the biome. Plants and Glowing Mushrooms can grow inside these gaps.
- Caution should be taken when creating a Surface Glowing Mushroom biome in pre-Hardmode, as the enemies that spawn there are much more powerful than other pre-Hardmode enemies.
- It is a wise idea to make two NPC houses in pre-Hardmode in a surface Glowing Mushroom biome in preparation for the Truffle and a companion NPC such as the Dryad. This will also address the issue listed above, as having NPCs in the biome will drastically reduce enemy spawn rates.
- On the Desktop version and Mobile version, either Surface or Underground Mushroom biomes will allow obtaining a Mushroom pylon, by building two or more houses and placing chosen NPCs there; it is not necessary to wait for Hardmode and Truffle's appearance.
- On the surface, it is easiest to produce the biome in the Jungle due to the prevalence of mud; this can provide a second pylon for the Jungle area. Alternatively, mud can simply be placed as needed. When choosing a location, consider that the Mushroom biome background will appear up to a screen's width or so away from the actual Mushroom grass etc. producing the biome.
- Alternatively, the natural Mushroom biomes will mostly appear at great depths, and these can be "colonized" to provide a convenient way-station to the Underworld. Naturally, the NPCs should be fully sealed away from the dangerous local enemies; even so, the spores of Fungi Bulbs and Giant Fungi Bulbs may be troublesome in Hardmode.
- Note that NPCs placed in an Underground Mushroom biome will probably qualify as a "Cavern Town", and spawn Gem Critters in the vicinity. These will look quite odd (and nearly indistinguishable) by the biome's natural lighting, but supplying some brighter lighting will show their various colors.
- Due to the red tint of the Blood Moon, Surface Glowing Mushroom biomes take on a pinkish purple hue when one occurs.
- The moon will always be red in a Surface Glowing Mushroom biome, similar to a Blood Moon.
- A sample from the 1990 side-scrolling platform game Super Mario World is used in the background music for the Glowing Mushroom biome.
- Underground Glowing Mushroom biomes will glow brighter if the player is near the "center" of the biome.
- When entering or leaving a Surface Glowing Mushroom biome, there is a noticeable delay as the sunlight fades to darkness, and especially when it returns.
- Painting only one Mud Block then using one Mushroom Grass Seed on it and letting it spread to adjacent Mud Blocks will allow to create a whole colored Glowing Mushroom biome. Normal paints will only be visible with a light source while deep paints will always be visible.
This content is transcluded from Biome backgrounds § Glowing Mushroom biome.
Glowing Mushroom backgrounds are combined with the current forest background.
- Desktop 184.108.40.206:
- Added three new backgrounds for the Surface Glowing Mushroom biome.
- Mushroom Chests in Underground Cabins now have a
50*1/2 (50%) chance of containing the Shroom Minecart and a 33.33*1/3 (33.33%) chance of containing the Mushroom vanity set. These will spawn as secondary loot alongside the loot from ordinary Gold Chests.
- Mushroom Grass Seeds now have a
2.5*1/40 (2.5%) chance of being dropped from destroyed Glowing Mushrooms.
- Spore Bats and Spore Skeletons now spawn in the Underground Glowing Mushroom biome at all stages of the game. The former has a
1*1/100 (1%) chance of dropping the new Mushroom-themed Shroomerang, while the latter drops all of the same loot as other types of Skeletons, including the new Carton of Milk.
- The Mushroom Pylon is now sold by any happy NPC in the Glowing Mushroom biome.
- Underground Glowing Mushroom world is now larger and redesigned, with hanging mushroom lamps, large lakes for fishing, new backgrounds, and surface biome trees. They also spawn much larger and in a layered formation.
- Desktop 220.127.116.11:
- Desktop 1.2.4: The very rare Truffle Worm critter, which is fished as bait to spawn the new Duke Fishron boss, now spawns in the Underground Glowing Mushroom biome in Hardmode.
- Desktop 1.2:
- Can now be spread with the Clentaminator using Dark Blue Solution on mud.
- Glowing Mushrooms now have their own biome with exclusive backgrounds, enemies, and music:
- Now can be cultivated as a Surface Mushroom biome on the surface:
- The new, Surface Mushroom biome-exclusive Truffle NPC will now move into any valid NPC house in a Surface Mushroom biome in Hardmode.
- The Dryad will now sell Mushroom Grass Seeds when in the Glowing Mushroom biome.
- Desktop-Release: Introduced.
- Console 1.0.933.1: Underground Glowing Mushroom cabins and furniture added. ()
- Console 1.0.750.0: Underground Glowing Mushroom cabins and furniture added. ()
- Console 1.02: Glowing Mushrooms given their own biome (music, background, spawns, etc.) and added the ability to cultivate one on the Surface.
- Console-Release: Introduced.
- Switch 1.0.711.6: Introduced with changes up to Desktop 18.104.22.168.
- Mobile 1.2.6508: Glowing Mushrooms given their own biome (music, background, spawns, etc.) and added the ability to cultivate one on the Surface.
- Mobile-Release: Introduced.
- 3DS-Release: Introduced with changes up to Desktop 1.2.