Larvae are created during world generation, and each world has a limited number of them. Once all Larvae in a world have been destroyed, Queen Bee can still be summoned via the Abeemination, a crafted item.
- The Clentaminator's beam will not destroy Larvae.
- Players who are unprepared to fight Queen Bee should be very cautious around Larvae, as they are very fragile. Similar to Pots and grass, a Larva is destroyed with a single hit from weapons or damaging tools. In addition, it is destroyed in the following ways:
- From hooks, even if latched from below onto the blocks the Larva is placed on.
- From any minion that deals contact damage.
- By creating a block on one of the tiles that are occupied by the Larva.
- From Fallen Stars in Not the bees world seed, where Larvae generate on the surface.
- A door opening on to a Larvae
- Even if the player is a ghost in hardcore mode, opening doors automatically will summon Queen Bee. However, if no players are actually alive Queen Bee will despawn.
- Breaking more than one Larvae at a time can spawn more than one Queen Bee, allowing the player to fight more Queen Bees at one time.
- This usually happens in the drunk world as hives spawn with more than one larvae. If not intended, proceed with caution when fighting so as to not break another Larvae by accident.
- Ores created when breaking a demon altar can generate on the blocks that the larva is on, but will not break it.
- Desktop 1.3.1: Sprite updated.
- Desktop 1.2: Introduced.
- Console 1.0.933.1: Sprite updated to match Desktop 1.3.1. ()
- Console 1.0.750.0: Sprite updated to match Desktop 1.3.1. ()
- Console 1.02: Introduced.
- Switch 1.0.711.6: Introduced.
- Mobile 1.2.6508: Introduced.
- 3DS-Release: Introduced.